ChaoPVA · Anticancer Chaotropic PVA Micelles with DNA-motif-mediated Targeting

In this project, I propose an innovative nanoscale therapeutic system for the optimized delivery of ellipticine (Elli), as a proof of concept, to cancer cells. Utilizing polyvinyl alcohol (PVA)—a biocompatible and biodegradable polymer—we will form micelles that encapsulate Elli within their hydrophobic core.To overcome challenges associated with cellular uptake and nanoparticle´s trafficking, we will functionalize the surface of the micelles with boron clusters to endow the nanocarrier with chaotropic activity, thereby facilitating enhanced membrane translocation. Further, we will integrate DNA motifs, specifically pyramid-shaped linkages (L-Py), into the boron cluster framework through coordination bonds. This modification is anticipated to permit selective targeting of cancer cells, thereby maximizing therapeutic efficacy while minimizing collateral damage to healthy tissues.The therapeutic efficacy of the resulting complex, formulated as Elli@APVA/n(L-Py)‒[B12Br12-n]2-, will be comprehensively evaluated using both in vitro 3D cell culture models and in vivo mimicking approaches, including 3D bioprinting.Overall, this project will allow me to carry out a 2-year project independently, growing towards a mature, professional, and independent researcher in the drug delivery field, reinforcing my expertise on advanced characterization of nanoparticle-cell interactions.